Close
  Indian J Med Microbiol
 

Figure 1: Detection of lipoprotein lipase (LPL) gene polymorphism: (a) LPL PvuII product: PvuII expected product length 430 bp. (b) PCR-REFLP analysis using LPL PvuII restriction enzyme analysis by 2% agarose gel electrophoresis. Lane 1: ladder (50 bp). Lanes 2, 3, 5, 6, 9, and 13: heterozygous (+,−) 430, 320, and 110 bp. Lanes 4, 7, 8, and 11: wild homozygous form has no restriction (−,−) 430 bp. Lanes 10 and 12 homozygous mutant form (+,+) 320 and 110 bp. (c) LPL S447X product: the expected length product is ∼488 bp. (d) LPL S447X RFLP product: 2% agarose gel electrophoresis cutting with MnlI. Lane 1: DNA molecular ladder (50 bp). Lane 2: wild homozygous 200 and 290 bp. Lane 3: heterozygosity 290, 250 and 200 bp.

Figure 1: Detection of lipoprotein lipase (LPL) gene polymorphism: (a) LPL <i>PvuII</i> product: <i>PvuII</i> expected product length 430 bp. (b) PCR-REFLP analysis using LPL <i>PvuII</i> restriction enzyme analysis by 2% agarose gel electrophoresis. Lane 1: ladder (50 bp). Lanes 2, 3, 5, 6, 9, and 13: heterozygous (+,−) 430, 320, and 110 bp. Lanes 4, 7, 8, and 11: wild homozygous form has no restriction (−,−) 430 bp. Lanes 10 and 12 homozygous mutant form (+,+) 320 and 110 bp. (c) LPL <i>S447X</i> product: the expected length product is ∼488 bp. (d) LPL <i>S447X</i> RFLP product: 2% agarose gel electrophoresis cutting with <i>Mnl</i>I. Lane 1: DNA molecular ladder (50 bp). Lane 2: wild homozygous 200 and 290 bp. Lane 3: heterozygosity 290, 250 and 200 bp.