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  Indian J Med Microbiol
 

Figure 1: Detection of wild-type codons, of 120 bp for codon 552 and 192 bp for codon 528, visualized under ultraviolet light. In codon 552, the mutation (CATATG) creating the NdeI restriction site resulted in 100+20-bp, and the mutation (CATGTG) creating the NlaIII site resulted in 99+21-bp bands. In codon 528, the mutation (CATG) creating the NlaIII restriction site resulted in 136+56-bp bands. Lane 1: 100-bp ladder; lane 2: mutant codon 552 (no digestion with NdeI); lane 3: heterozygote variation of Val/Iso (partial digestion with NlaIII); lane 4: undigested amplified codon 552; lane 5: double population of codon 528 (Met/Leu) and heterozygote mutation of codon 528 (partial digestion with NlaIII); lane 6: undigested amplified codon 528.

Figure 1: Detection of wild-type codons, of 120 bp for codon 552 and 192 bp for codon 528, visualized under ultraviolet light. In codon 552, the mutation (CATATG) creating the <i>Nde</i>I restriction site resulted in 100+20-bp, and the mutation (CATGTG) creating the <i>Nla</i>III site resulted in 99+21-bp bands. In codon 528, the mutation (CATG) creating the <i>Nla</i>III restriction site resulted in 136+56-bp bands. Lane 1: 100-bp ladder; lane 2: mutant codon 552 (no digestion with <i>Nde</i>I); lane 3: heterozygote variation of Val/Iso (partial digestion with <i>Nla</i>III); lane 4: undigested amplified codon 552; lane 5: double population of codon 528 (Met/Leu) and heterozygote mutation of codon 528 (partial digestion with <i>Nla</i>III); lane 6: undigested amplified codon 528.