Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2020  |  Volume : 33  |  Issue : 1  |  Page : 231-235

Isolation and osteogenic differentiation of umbilical cord mesenchymal stem cells


1 Department of Clinical Pathology, Faculty of Medicine, Menoufia University, Shiben El-Kom, Egypt
2 Clinical Pathology Department, National Liver Institute, Menoufia University, Shiben El-Kom, Egypt
3 Department of Obstetrics and Gynecology, Faculty of Medicine, Menoufia University, Shiben El-Kom, Egypt

Correspondence Address:
Asmaa Z.A. Turky
Shiben El.Kom, Menoufia
Egypt
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/mmj.mmj_265_18

Rights and Permissions

Objective To investigate the isolation and in-vitro differentiation of umbilical cord mesenchymal stem cells (MSC) into osteocytes. Background Umbilical cord is an important source of multiple cell types such as MSCs that possess the ability of self-renewal and differentiation into several cell types including osteocytes. Bone healing is a complex process that can eventually lead to the formation of malunions, delayed unions, and osteomyelitis. Cellular therapy and bone tissue engineering are becoming a useful addition to medical therapies for bone repairing and restoring function which is a potential tool for the treatment of multiple bone diseases. Patients and methods The present experimental study was conducted at Clinical Pathology, Obstetrics and Gynecology Departments, Menoufia University Hospital from May 2017 to April 2018 after obtaining Menoufia Ethical Committee approval. The study was carried out on 35 umbilical cord samples. Wharton's jelly separated from the umbilical cord was divided into small pieces and cultured in tissue culture plastic flasks. MSCs were separated by plastic adherence, then subcultured in Petri dish 35 mm, treated with osteogenic differentiation media and identified by morphology and immunocytochemistry. Results MSCs were isolated from Wharton's jelly of the umbilical cord. Isolated MSCs were positive for the markers cluster of differentiation (CD) 44 and CD73 and negative for CD34. They were able to differentiate into osteocytes. Conclusion The present study showed that MSCs can be isolated from Wharton's jelly of the human umbilical cord and can be differentiated into osteocytes.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed241    
    Printed5    
    Emailed0    
    PDF Downloaded35    
    Comments [Add]    

Recommend this journal