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ORIGINAL ARTICLE
Year : 2017  |  Volume : 30  |  Issue : 4  |  Page : 1072-1078

Urinary 8-hydroxy-2′-deoxyguanosine as an oxidative DNA damage biomarker in chronic heart failure


1 Department of Medical Biochemistry, Menoufia University, Menoufia, Egypt
2 Department of Cardiology, Menoufia University, Menoufia, Egypt

Correspondence Address:
Sara K Rizk
Department of Medical Biochemistry, Menoufia University, Shebin Elkom, Menoufia
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/mmj.mmj_700_16

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Objective The aim of this study was to evaluate the role of urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) as a biomarker of oxidative DNA damage in patients with chronic heart failure (CHF). Background CHF is the leading cause of morbidity, mortality, and increasing healthcare costs around the world. Oxidative stress is known to play a crucial role in the pathogenesis of heart failure. In nuclear and mitochondrial DNA, 8-OHdG is one of the predominant forms of free radical-induced oxidative lesions. Patients and methods This study was conducted on 80 individuals: 50 CHF patients (22 men and 28 women) and 30 healthy controls (16 men and 14 women). The patients were selected from the Cardiology Department of Menoufia University Hospital during the period June 2015 to December 2015. All individuals included in this study were subjected to full history taking, clinical examination, echocardiography, and laboratory investigations that included tests for evaluation of serum lipid profiles, fasting blood sugar, 2 h postprandial glucose, serum creatinine, malondialdehyde, catalase, and urinary 8-OHdG, which was carried out by enzyme-linked immunosorbent assay technique. Results Urinary 8-OHdG was significantly higher in patients than in controls. There was a significant statistical increase in malondialdehyde in patients compared with controls. There was a significant statistical decrease in catalase in patients compared with controls. There was a significant negative correlation between urinary 8-OHdG and systolic ejection fraction in the patients' group. Urinary 8-OHdG can be used as a biomarker in CHF at a cutoff of 5.5 ng/mg creatinine with an accuracy of 87.5%. Conclusion In CHF, urinary 8-OHdG can be a reliable marker of DNA damage as it reflects the level of oxidative stress and degree of CHF severity.


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